04 - 08 Jun 2018

EADV-ESDR Summer Research Workshop - Advanced molecular biology tools in dermatological research

This summer school is specifically aimed at dermatology residents/trainees and/or at PhD students and postdocs affiliated to dermatological research with limited experience in experimental research and in molecular biology.

The workshop will comprise a mixture of research presentations, interactive and practical sessions. A booklet of methods and protocol in advanced molecular biology will be given to participants during the course. Topics covered will include:

 

Gene transduction and silencing in epidermal cells:

  • isolation of human and mouse primary keratinocytes
  • keratinocyte culture and keratinocyte/fibroblast co-culture
  • efficient transduction of genes or gene knockdown in keratinocytes

 

Gene expression in skin in health and disease:

  • RNA isolation from human and mouse primary keratinocytes, from freshly isolated epidermis, and cells of interest from fixed skin
  • quantitative RT-PCR: procedure and data analysis
  • RNA sequencing and data analysis

 

Genomic editing:

  • designing of gene deletion or gene replacement by CRISPR/Cas9
  • preparation of Cas9-guide RNA vectors
  • assess genome editing efficiency
  • isolation of genome-edited cells

Where

Naples, Italy

Terms

EADV Resident courses are for dermatology and venereology residents (both EADV members and non EADV members).

Selected candidates will receive free registration and the educational material which was presented during the course. EADV also offers accommodation, catering and a social event during the course.

The selection of candidates is made based on CVs, year of residency and the amount of courses previously attended.

The registration deadline will close at 23h59 (GMT+1) the day before the registration deadline (ex: registration deadline 15 January 2018 – the registrations will close on 14 January 2018 at 23h59).

Programme

Monday 4 June 2018

  • 08:30 / 09:00 Welcome and overview of the course in advanced molecular biology of the skin
  • 09:00 / 10:00 Introductory lecture: "Advanced molecular biology in dermatological research: a clinician's perspective"
  • 10:00 / 10:30 Break and refreshments
  • 10:30 / 11:45 Isolation and culture of human and mouse primary epidermal cells (monolayer, organotypic, co-culture with fibroblasts)
  • 11:45 / 13:00 Quantitative analysis of gene expression in skin cells
  • 13:00 / 14:00 Lunch
  • 14:00 / 15:00 Participant presentation (10' each + 5' Q&A)
  • 15:00 / 18:30 Practical session: Co-culture of human keratinocytes and fibroblasts, isolation of mouse keratinocytes; RNA isolation from keratinocytes and epidermis, cDNA preparation

Tuesday 5 June 2018

  • 08:30 / 09:30 Lecture: "Mouse models for inherited diseases of the skin"
  • 09:30 / 10:30 iPS isolation and reprogramming in keratinocytes
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 11:30 Fibroblast to keratinocyte conversion assay
  • 11:30 / 12:15 Gene delivery and gene knockdown in epidermal cells
  • 12:15 / 13:00 RNA sequencing techniques and analysis
  • 13:00 / 14:00 Lunch
  • 14:00 / 15:00 Participant presentation (10' each + 5' Q&A)
  • 15:00 / 18:30 Practical session: Gene delivery and gene knockdown in epidermal cells and qRT-PCR set-up and analysis

Wednesday 6 June 2018

  • 08:30 / 09:30 Lecture: "Single-Cell Transcriptomics Reveals Heterogeneity in epidermis and hair follicle"
  • 09:30 / 10:30 TBA
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 12:00 TBA
  • 12:00 / 13:00 TBA
  • 13:00 / 14:00 Lunch
  • 14:00 / 15:00 Participant presentation (10' each + 5' Q&A)
  • 15:00 / 18:30 Practical session: Preparation of samples for RNA sequencing and bioinformatics analysis of gene networks and signaling pathways obtained by RNA sequencing

Thursday 7 June 2018

  • 08:30 / 09:30 Lecture: "Basis of Cancer Evolution and Field Cancerization"
  • 09:30 / 10:30 Basic principles of genome editing
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 12:00 Designing of gene deletion or gene replacement by CRISPR/Cas9
  • 12:00 / 13:00 Genome editing in primary keratinocytes
  • 13:00 / 14:00 Lunch
  • 14:00 / 15:00 Participant presentation (10' each + 5' Q&A)
  • 15:00 / 18:00 Practical session: designing strategy of gene deletion or gene replacement by CRISPR/Cas9 and assessment of genome editing efficiency
  • 20:00 / 22:00 Dinner with participants

Friday 8 June 2018

  • 08:30 / 09:30 Lecture: "Contribution of thyroid hormone metabolism on non melanoma skin cancer"
  • 09:30 / 10:30 Participant presentation (10' each + 5' Q&A)
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 12:00 Gene correction in skin
  • 12:00 / 13:00 Closing discussion
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