04 - 08 Jun 2018

EADV-ESDR Summer Research Workshop - Advanced molecular biology tools in dermatological research

This summer school is specifically aimed at dermatology residents/trainees and/or at PhD students and postdocs affiliated to dermatological research with limited experience in experimental research and in molecular biology.

The workshop will comprise a mixture of research presentations, interactive and practical sessions. A booklet of methods and protocol in advanced molecular biology will be given to participants during the course. Topics covered will include:

 

Gene transduction and silencing in epidermal cells:

  • isolation of human and mouse primary keratinocytes and melanocytes
  • keratinocyte culture and keratinocyte/fibroblast co-culture
  • efficient transduction of genes or gene knockdown in keratinocytes

 

Gene expression in skin in health and disease:

  • RNA isolation from human and mouse primary keratinocytes, from freshly isolated epidermis, and cells of interest from fixed skin
  • Quantitative RT-PCR: procedure and data analysis
  • RNA sequencing and data analysis
  • Basis of epigenetic control of gene expression

 

Genomic editing:

  • Designing of gene deletion or gene replacement by CRISPR/Cas9
  • Preparation of Cas9-guide RNA vectors
  • Assess genome editing efficiency
  • Isolation of genome-edited cells

Where

Naples, Italy

Terms

EADV Resident courses are for dermatology and venereology residents (both EADV members and non EADV members).

Selected candidates will receive free registration and the educational material which was presented during the course. EADV also offers accommodation, catering and a social event during the course.

The selection of candidates is made based on CVs, year of residency and the amount of courses previously attended.

The registration deadline will close at 23h59 (GMT+1) the day before the registration deadline (ex: registration deadline 15 January 2018 – the registrations will close on 14 January 2018 at 23h59).

Programme

Monday 4 June 2018

  • 08:30 / 09:00 Welcome and overview of the course in advanced molecular biology of the skin   -   C. Missero
  • 09:00 / 09:45 Introductory lecture: Advanced molecular biology in dermatological research: a clinician's perspective   -   M. Picardo (ESDR President 2015)
  • 09:45 / 10:30 Human organotypic cultures and their applications in pathophysiology of the skin   -   A. Balato
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 11:45 Isolation and culturing of melanocytes   -   L. Larue
  • 11:45 / 12:30 Organization of the practical session and lab safety
  • 12:30 / 13:00 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 13:00 / 14:00 Lunch
  • 14:00 / 14:30 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 14:30 / 18:30 Practical session: Skin organotypic culture, UV irradiation. Isolation of human keratinocytes and plating of feeder cells. Isolation of mouse keratinocytes. RNA isolation from keratinocytes and epidermis.

Tuesday 5 June 2018

  • 09:00 / 09:45 Keratinocyte differentiation and the epidermal barrier to the environment   -   L. Eckhart
  • 09:45 / 10:30 Quantitative analysis of gene expression in skin cells   -   D. Antonini
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 11:45 Transcriptomics in mouse epidermis   -   C. Missero
  • 11:45 / 12:30 Gene delivery and gene knockdown in epidermal cells   -   D. Antonini
  • 12:30 / 13:00 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 13:00 / 14:00 Lunch
  • 14:00 / 14:30 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 14:30 / 18:30 Practical session: Second day of mouse primary keratinocyte isolation. Culture of human keratinocytes on feeders. cDNA and qRT-PCR set-up. Gene delivery and gene knockdown in epidermal cells

Wednesday 6 June 2018

  • 09:00 / 09:45 Tumor cell lineage-specific molecular signatures in melanoma   -   L. Larue
  • 09:45 / 10:30 Epigenetics: principle and practice   -   J. H. Zhou
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 11:45 Epigenetics and transcriptomics in human keratinocytes   -   J. H. Zhou
  • 11:45 / 12:30 iPS and fibroblast reprogramming in keratinocytes   -   D. Cacchiarelli
  • 12:30 / 13:00 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 13:00 / 14:00 Lunch
  • 14:00 / 14:30 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 14:30 / 18:30 Practical session: Analysis of gene expression data. Assessment of gene delivery efficiency and purification of genetically-modified cells by cell sorting. Bioinformatics analysis of gene networks and signaling pathways obtained by RNA sequencing

Thursday 7 June 2018

  • 09:00 / 09:45 In vitro and in vivo models of genodermatoses   -   D. Kelsell (ESDR President-elect)
  • 09:45 / 10:30 Basic principles and applications of genome editing   -   H. Bazzi
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 11:45 Studying autophagy in skin cells   -   E. Tschachler (EADV President 2014-2016)
  • 11:45 / 12:30 Genome editing in primary keratinocytes and in skin   -   C. Missero
  • 12:30 / 13:00 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 13:00 / 14:00 Lunch
  • 14:00 / 14:30 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 14:30 / 18:00 Practical session: Epigenetic signature of human keratinocytes. Designing strategy of gene deletion or gene replacement by CRISPR/Cas9 and assessment of genome editing efficiency
  • 20:00 / 22:00 Dinner with participants

Friday 8 June 2018

  • 09:00 / 09:45 Mouse models for inherited diseases of the skin   -   C. Missero
  • 09:45 / 10:30 RNA sequencing techniques and analysis   -   D. Cacchiarelli
  • 10:30 / 11:00 Break and refreshments
  • 11:00 / 11:45 Transcriptomic analysis of psoriasis following UVB and biologic therapy   -   N. Reynolds (ESDR President 2014)
  • 11:45 / 12:30 Participants' presentations (research presentation or journal club; 10' each + 5' Q&A)
  • 12:30 / 13:00 Closing discussion   -   N. Reynolds (ESDR President 2014)
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